Does Acetaminophen Deplete Glutathione? Understanding the Connection Cymbiotika CYMBIOTIKA How Glutathione is Depleted from the Body Suzy Cohen, RPh offers natural remedies to help you feel better now! What is Glutathione Agarose? GoldBio Purification Using GST HiTrap 1 mL and 5 mL Columns: GSTrap 4B, GSTrap FF, and GSTrap HP Acetaminophen hepatotoxicity Clinics in Liver Disease Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.